Description
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with D2D. During the reaction, D2D in the sample or standard competes with a fixed amount of D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to D2D. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of D2D in the samples is then determined by comparing the OD of the samples to the standard curve